(PDF) gRNA Design and Cloning With BbsI Into Loop Plasmid

gRNA Design and Cloning With BbsI Into Loop Plasmid L1_lacZgRNA-Ck2/3 V2 (protocols.io.bc6fizbn)

doi 10.17504/protocols.io.bc6fizbn

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Date

March 3, 2020

Authors

Eftychis Frangedakis

marta tomaselli

Susana Sauret

Publisher

ZappyLab, Inc.

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gRNA Design and Cloning With BbsI Into Loop Plasmid L1_lacZgRNA-Ck2/3 V2 (protocols.io.bc6fizbn)

doi 10.17504/protocols.io.bc6fizbn

Full Text

Abstract

Date

Authors

Publisher

Related search

Stabilization of the V2 Loop Improves the Presentation of V2 Loop–associated Broadly Neutralizing Antibody Epitopes on HIV-1 Envelope Trimers

Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion Into Any Target Plasmid Without Sequence Constraints

Molecular Epidemiology of the Protective RV144 V2 Loop Epitope

An Optimized Recipe for Cloning of the Polymerase Chain Reaction-Amplified DNA Inserts Into Plasmid Vectors.

Identification of High-Efficiency 3′GG gRNA Motifs in Indexed FASTA Files With Ngg2

Unified Energetics Analysis Unravels SpCas9 Cleavage Activity for Optimal gRNA Design

Bovine Papilloma Viral Gene Transfection Into Human Hepatoma Cells: Cloning and Analysis of the Plasmid Replicating in the Transformant.

Cloning and Analysis of a Large Plasmid pBMB165 From Bacillus Thuringiensis Revealed a Novel Plasmid Organization

Characterizing the Convergence of Protein Kinase CK2 and Caspase-3 Reveals Isoform-Specific Phosphorylation of Caspase-3 by CK2&alpha;&prime;: Implications for Pathological Roles of CK2 in Promoting Cancer Cell Survival.

Characterizing the Convergence of Protein Kinase CK2 and Caspase-3 Reveals Isoform-Specific Phosphorylation of Caspase-3 by CK2α′: Implications for Pathological Roles of CK2 in Promoting Cancer Cell Survival.